Anti-Human DPEP3 Recombinant Antibody (B43), APC (HV426137)

Name: Anti-Human DPEP3 Recombinant Antibody (B43), APC
Brand: abinScience
SKU: HV426137
Price: 290 USD
Availability: InStock

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Número de catálogo	HV426137
Reactividad de especies	Human
Aplicaciones	ELISA, FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	B43
Conjugación	APC
Objetivo	DPEP3, Dipeptidase 3
Nivel de endotoxinas	Please contact with the lab for this information.
Productos asociados	Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
Purificación	Affinity chromatography.
Número de acceso	Q9H4B8
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance. 1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD19 antibody monoclonal antibody (Catalog # HV426137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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