Anti-Human DPEP3 Recombinant Antibody (B43), APC (HV426137)

Name: Anti-Human DPEP3 Recombinant Antibody (B43), APC
Brand: abinScience
SKU: HV426137
Price: 290 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HV426137
種反応性	Human
アプリケーション	ELISA, FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG2a, kappa
クローンID	B43
抱合	APC
ターゲット	DPEP3, Dipeptidase 3
エンドトキシンレベル	Please contact with the lab for this information.
関連製品	Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
精製	Affinity chromatography.
アクセッション番号	Q9H4B8
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance. 1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD19 antibody monoclonal antibody (Catalog # HV426137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート