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Anti-Human DPEP3 Recombinant Antibody (B43), APC
Anti-Human DPEP3 Recombinant Antibody (B43), APC
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Спецификация:
  • 50T
  • 100T
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHV426137
Реактивность видовHuman
ПримененияELISA, FCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG2a, kappa
ID клонаB43
КонъюгацияAPC
Цель DPEP3, Dipeptidase 3
Уровень эндотоксинов Please contact with the lab for this information.
Связанные продукты Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
Очистка Affinity chromatography.
Номер доступа Q9H4B8
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance.

1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Human DPEP3 Recombinant Antibody (B43), APC

    Flow Cytometry

    Flow-cytometry using APC anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD19 antibody monoclonal antibody (Catalog # HV426137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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