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Anti-Human EWSR1 Polyclonal Antibody (HX938014)

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Vista general
Número de catálogoHX938014
Descripción
Anti-Human EWSR1 Polyclonal Antibody (HX938014) is a rabbit polyclonal antibody detecting EWSR1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human EWSR1 (Asp353-Arg446).
Objetivo RNA-binding protein EWS, EWS oncogene, EWS, EWSR1, Ewing sarcoma breakpoint region 1 protein
Purificación Purified by antigen affinity column.
Número de acceso Q01844
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

RNA-binding protein EWS (EWSR1) is a ~68 kDa protein. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

1. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132
2. Li, KK. et al. (2000) The Journal of biological chemistry 275, 23053-8. PMID: 10767297
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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