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Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440) (HF916023)

Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
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Aperçu
Numéro de catalogueHF916023
Description
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440) (HF916023) is a human monoclonal antibody detecting Coagulation Factor IX in ELISA, Bioactivity: FCM, Functional assay, Research in vivo. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, Bioactivity: FCM, Functional assay, Research in vivo
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG4, kappa
ID de cloneSAA2440
Cible Coagulation factor IX, EC:3.4.21.22, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9
Niveau d'endotoxines < 10 EU/mg
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P00740
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Coagulation factor IX (F9) is a ~51 kDa protein. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

1. de la Salle, C. et al. (1993) Nouvelle revue francaise d'hematologie 35, 473-80. PMID: 8295821
2. Suehiro, K. et al. (1989) The Journal of biological chemistry 264, 21257-65. PMID: 2592373
3. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1473-82. PMID: 20121197
4. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1465-72. PMID: 20121198
5. Rawala-Sheikh, R. et al. (1992) Blood 79, 398-405. PMID: 1730085
6. Simioni, P. et al. (2009) The New England journal of medicine 361, 1671-5. PMID: 19846852
7. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037
Note For research use only
Isotype Control Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207)
Images
  • Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)

    SDS-PAGE

    SDS-PAGE for Anti-Human F9/Factor IX Antibody (SAA2440)

  • Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)

    Flow cytometry

    Flow-cytometry using anti-human F9 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F9 monoclonal antibody (Catalog HF916023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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