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Anti-Human F9/Factor IX Recombinant Antibody (10C12) (HF916013)

Anti-Human F9/Factor IX Recombinant Antibody (10C12)
Anti-Human F9/Factor IX Recombinant Antibody (10C12)
Anti-Human F9/Factor IX Recombinant Antibody (10C12)
Anti-Human F9/Factor IX Recombinant Antibody (10C12)
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Overview
Catalog No.HF916013
Species reactivityHuman
ApplicationsBlocking, ELISA
Host speciesMouse
IsotypeIgG2a, kappa
Clone ID10C12
Clonality Monoclonal
Target Coagulation factor IX, 3.4.21.22, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P00740
RRID Anti-Human F9/Factor IX Antibody (10C12) (abinScience Cat# HF916013, RRID:AB_3729526)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Coagulation factor IX (F9) is a ~51 kDa protein. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

1. de la Salle, C. et al. (1993) Nouvelle revue francaise d'hematologie 35, 473-80. PMID: 8295821
2. Suehiro, K. et al. (1989) The Journal of biological chemistry 264, 21257-65. PMID: 2592373
3. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1473-82. PMID: 20121197
4. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1465-72. PMID: 20121198
5. Rawala-Sheikh, R. et al. (1992) Blood 79, 398-405. PMID: 1730085
6. Simioni, P. et al. (2009) The New England journal of medicine 361, 1671-5. PMID: 19846852
7. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037
Note For research use only.
Images
  • Anti-Human F9/Factor IX Recombinant Antibody (10C12)

    SDS-PAGE

    SDS-PAGE for Anti-Human F9/Factor IX Antibody (10C12)

  • Anti-Human F9/Factor IX Recombinant Antibody (10C12)

    Bioactivity

    Detects recombinant human F9/Factor IX (Catalog No: HF916011) in indirect ELISA

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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