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Anti-Human FBXO45 Polyclonal Antibody (HY575014)

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Anti-Human FBXO45 Polyclonal Antibody
Anti-Human FBXO45 Polyclonal Antibody
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Vista general
Número de catálogoHY575014
Descripción
Anti-Human FBXO45 Polyclonal Antibody (HY575014) is a rabbit polyclonal antibody detecting FBXO45 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human FBXO45 (Ser41-Gly286).
Objetivo F-box/SPRY domain-containing protein 1, F-box only protein 45, hFbxo45, FBXO45, FBX45
Purificación Purified by antigen affinity column.
Número de acceso P0C2W1
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

F-box/SPRY domain-containing protein 1 (FBXO45) is a ~30 kDa protein. Component of E3 ubiquitin ligase complex consisting of FBXO45, MYCBP2 and SKP1. Functions in substrate recognition but also plays an important role in assembly of the complex. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Regulates neuron migration during brain development through interaction with N-cadherin/CDH2 after secretion via a non-classical mechanism. Plays a role in the regulation of neurotransmission at mature neurons.

1. Desbois, M. et al. (2018) The Journal of biological chemistry 293, 13897-13909. PMID: 29997255
2. Richter, KT. et al. (2020) Cell death and differentiation 27, 758-772. PMID: 31285543
3. Tsai, M. et al. (2022) The Journal of biological chemistry 298, 102698. PMID: 36379255
Nota For research use only.
Imágenes
  • Anti-Human FBXO45 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FBXO45 polyclonal antibody at FBXO45 on various samples.
    Lane 1: Mouse brain lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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