Human FBXO45 Recombinant Protein (N-His) (HY575012)

Name: Human FBXO45 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY575012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY575012
Description	Recombinant Human FBXO45 Protein, N-His (HY575012) expressed in E. coli, spanning Ser41-Gly286. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0C2W1
Protein length	Ser41-Gly286
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.95 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	F-box/SPRY domain-containing protein 1, F-box only protein 45, hFbxo45, FBXO45, FBX45
Background	F-box/SPRY domain-containing protein 1 (FBXO45) is a ~30 kDa protein. Component of E3 ubiquitin ligase complex consisting of FBXO45, MYCBP2 and SKP1. Functions in substrate recognition but also plays an important role in assembly of the complex. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Regulates neuron migration during brain development through interaction with N-cadherin/CDH2 after secretion via a non-classical mechanism. Plays a role in the regulation of neurotransmission at mature neurons. 1. Desbois, M. et al. (2018) The Journal of biological chemistry 293, 13897-13909. PMID: 29997255 2. Richter, KT. et al. (2020) Cell death and differentiation 27, 758-772. PMID: 31285543 3. Tsai, M. et al. (2022) The Journal of biological chemistry 298, 102698. PMID: 36379255
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human FBXO45 Protein, N-His

References

Datasheet

Human FBXO45 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human FBXO45 Recombinant Protein (N-His) (HY575012)

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