Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12) (HY272023)

Name: Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12)
Brand: abinScience
SKU: HY272023
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY272023

Reactividad de especies

Human

Aplicaciones

ELISA, FCM

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

8A12

Objetivo

HLBP14, Lactose-binding lectin 1, LGALS1, HBL, HPL, Beta-galactoside-binding lectin L-14-I, Gal-1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, Galaptin, Galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P09382

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Galectin-1 (Gal-1) is a ~14 kDa protein. Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896

Nota

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Imágenes

Bioactivity

Detects Human Gal1/LGALS1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human Gal1/LGALS1 Antibody (8A12).
Flow cytometry

Flow-cytometry using anti-human Galectin-1 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 monoclonal antibody (Catalog HY272023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.