Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12) (HY272023)

Name: Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12)
Brand: abinScience
SKU: HY272023
Price: 126 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY272023

種反応性

Human

アプリケーション

ELISA, FCM

宿主種

Mouse

クローン性

Monoclonal

アイソタイプ

IgG2a, kappa

クローンID

8A12

ターゲット

HLBP14, Lactose-binding lectin 1, LGALS1, HBL, HPL, Beta-galactoside-binding lectin L-14-I, Gal-1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, Galaptin, Galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P09382

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

背景

Galectin-1 (Gal-1) is a ~14 kDa protein. Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896

注意事項

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

イメージ

Bioactivity

Detects Human Gal1/LGALS1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human Gal1/LGALS1 Antibody (8A12).
Flow cytometry

Flow-cytometry using anti-human Galectin-1 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 monoclonal antibody (Catalog HY272023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.