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| カタログ番号 | HY030045 |
|---|---|
| 説明 |
Anti-Human GAPDH Monoclonal Antibody (1A201) [1A201] (HY030045) is a mouse monoclonal antibody detecting GAPDH in ELISA, WB. Suitable for Human, Mouse, and Rat.
Highlights
|
| 種反応性 | Human, Mouse, Rat |
| アプリケーション | ELISA, WB |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG2b |
| クローンID | 1A201 |
| 免疫原 | E. coli - derived recombinant Human GAPDH protein (Met1-Glu335). |
| ターゲット | GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. 1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794 2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585 3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094 4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158 5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501 |
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody (1A201)
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