Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310023)

Name: Anti-Human Glypican 3 Recombinant Antibody (YP7)
Brand: abinScience
SKU: HW310023
Price: 110 USD
Availability: InStock

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50ug
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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW310023

Reactivité des espèces

Human, Mouse

Applications

ELISA, FCM, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

YP7

Applications testées

WB, FCM

Cible

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P51654

RRID

Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310023, RRID:AB_3727765)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Note

For research use only.

Isotype Control

Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

Images

Bioactivity

Detects Human GPC3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: MW310011)
Lane 2: negative control
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate