Mouse GPC3/Glypican 3 Recombinant Protein (C-His) (MW310011)

Name: Mouse GPC3/Glypican 3 Recombinant Protein (C-His)
Brand: abinScience
SKU: MW310011
Price: 264 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	MW310011
Description	Recombinant Mouse GPC3/Glypican 3 Protein, C-His (MW310011) expressed in Insect Cells, spanning Met1-Ser553. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect cells
Accession	Q8CFZ4
Protein length	Met1-Ser553
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	63.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glypican-3, Glypican-3 alpha subunit, Glypican-3 beta subunit, Gpc3
Background	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. Capurro, MI. et al. (2008) Developmental cell 14, 700-11. PMID: 18477453 2. Bhave, VS. et al. (2013) The American journal of pathology 183, 153-9. PMID: 23665349 3. Song, HH. et al. (2005) The Journal of biological chemistry 280, 2116-25. PMID: 15537637 4. Paine-Saunders, S. et al. (2000) Developmental biology 225, 179-87. PMID: 10964473 5. Grisaru, S. et al. (2001) Developmental biology 231, 31-46. PMID: 11180950 6. Ng, A. et al. (2009) Developmental biology 335, 208-15. PMID: 19733558
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Mouse GPC3/Glypican 3 Protein, C-His
Bioactivity

Detected by Rat Anti-Human GPC3 Antibody (YP7) (Cat No.: HW310053) inindirectELISA.
Western blot

Detected by Anti-GPC3/Glypican monoclonal antibody (Catalog No: HW310023)