Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310023)

Name: Anti-Human Glypican 3 Recombinant Antibody (YP7)
Brand: abinScience
SKU: HW310023
Price: 110 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HW310023

Реактивность видов

Human, Mouse

Применения

ELISA, FCM, WB

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

YP7

Проверенные приложения

WB, FCM

Цель

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P51654

RRID

Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310023, RRID:AB_3727765)
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Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Примечание

For research use only.

Isotype Control

Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

Изображения

Bioactivity

Detects Human GPC3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: MW310011)
Lane 2: negative control
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate