Anti-Human Granzyme B Recombinant Antibody (SAA0515) (HY574013)

Name: Anti-Human Granzyme B Recombinant Antibody (SAA0515)
Brand: abinScience
SKU: HY574013
Price: 126 USD
Availability: InStock

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Spécification :

20ug
50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY574013

Reactivité des espèces

Human

Applications

ELISA, WB

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

SAA0515

Cible

Granzyme-B, Cathepsin G-like 1, Granzyme-2, grzmB, GZMB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P10144

RRID

Anti-Human GZMB Antibody (SAA0515) (abinScience Cat# HY574013, RRID:AB_3725204)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis.

1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927

2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682

3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427

4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716

5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257

6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940

7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Images

Bioactivity

SEC-HPLC detection for Anti-Human GZMB Antibody (ABS0372).
SDS-PAGE

SDS-PAGE for Anti-Human GZMB Antibody (SAA0515).
Western blot

Western blot analysis was performed using anti-GZMB monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GZMB (Catalog No: HY574012)
Lane 2: negative control