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Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345) (HY584023)

Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)
Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)
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Aperçu
Numéro de catalogueHY584023
Reactivité des espècesHuman
ApplicationsELISA, WB
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1
ID de cloneSAA2345
Cible Histidine--tRNA ligase cytoplasmic, EC:6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS, jo-1
Niveau d'endotoxines < 10 EU/mg
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P12081
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Histidine--tRNA ligase, cytoplasmic (HARS1/jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198
2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516
Note For research use only
Images
  • Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)

    Western blot

    Western blot analysis was performed using anti-HARS1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: HCT116 cell lysate
    Lane 6: Jurkat cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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