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| カタログ番号 | HY584023 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, WB | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1 | ||||||
| クローンID | SAA2345 | ||||||
| ターゲット | Histidine--tRNA ligase cytoplasmic, EC:6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS, jo-1 | ||||||
| エンドトキシンレベル | < 10 EU/mg | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P12081 | ||||||
| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 背景 | Histidine--tRNA ligase, cytoplasmic (HARS1/jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance. 1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198 2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516 | ||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-HARS1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT116 cell lysate
Lane 6: Jurkat cell lysate
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