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Anti-Human HDGFL2 Polyclonal Antibody (HA200014)

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Aperçu
Numéro de catalogueHA200014
Description
Anti-Human HDGFL2 Polyclonal Antibody (HA200014) is a rabbit polyclonal antibody detecting HDGFL2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human HDGFL2 (Met1-Asn87).
Cible Hepatoma-derived growth factor-related protein 2, HDGF-related protein 2, HRP-2, Hepatoma-derived growth factor 2, HDGF-2, HDGFL2, HDGF2, HDGFRP2, HRP2, UNQ785/PRO1604
Purification Purified by antigen affinity column.
Numéro d'accès Q7Z4V5
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Hepatoma-derived growth factor-related protein 2 (HDGFL2) is a ~74 kDa protein. Acts as an epigenetic regulator of myogenesis in cooperation with DPF3a (isoform 2 of DPF3/BAF45C). Associates with the BAF complex via its interaction with DPF3a and HDGFL2-DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of SMARCA4/BRG1/BAF190A (ATPase subunit of the BAF complex) to myogenic gene promoters. Promotes the repair of DNA double-strand breaks (DSBs) through the homologous recombination pathway by facilitating the recruitment of the DNA endonuclease RBBP8 to the DSBs. Preferentially binds to chromatin regions marked by H3K9me3, H3K27me3 and H3K36me2. Involved in cellular growth control, through the regulation of cyclin D1 expression.

1. Zhu, X. et al. (2020) Nucleic acids research 48, 6563-6582. PMID: 32459350
2. Baude, A. et al. (2016) Nucleic acids research 44, 2214-26. PMID: 26721387
3. Gao, K. et al. (2015) Biochemical and biophysical research communications 458, 849-55. PMID: 25689719
Note For research use only.
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Reférences
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human HDGFL2 Polyclonal Antibody

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