Anti-Human HIF1A Polyclonal Antibody (HS858014)

Name: Anti-Human HIF1A Polyclonal Antibody
Brand: abinScience
SKU: HS858014
Price: 168 USD
Availability: InStock

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概要
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参考文献
データシート
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概要

カタログ番号

HS858014

説明

Anti-Human HIF1A Polyclonal Antibody (HS858014) is a rabbit polyclonal antibody detecting HIF1A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human HIF1A (Asp55-Val342).

ターゲット

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8

精製

Purified by antigen affinity column.

アクセッション番号

Q16665

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Hypoxia-inducible factor 1-alpha (HIF1A) is a ~92 kDa protein. Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300.

1. Jaakkola, P. et al. (2001) Science (New York, N.Y.) 292, 468-72. PMID: 11292861

2. Masson, N. et al. (2001) The EMBO journal 20, 5197-206. PMID: 11566883

3. Bae, SH. et al. (2004) Biochemical and biophysical research communications 324, 394-400. PMID: 15465032

4. Choi, SM. et al. (2006) The Journal of biological chemistry 281, 34056-63. PMID: 16973622

5. Berta, MA. et al. (2007) Biochemical and biophysical research communications 360, 646-52. PMID: 17610843

6. Kim, EJ. et al. (2008) Arteriosclerosis, thrombosis, and vascular biology 28, 1796-802. PMID: 18658046

7. Gimm, T. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4443-58. PMID: 20624928

8. Shan, B. et al. (2012) Endocrine-related cancer 19, 13-27. PMID: 22009797

9. Xu, C. et al. (2018) PLoS pathogens 14, e1007259. PMID: 30125331

10. Bhattacharya, S. et al. (1999) Genes & development 13, 64-75. PMID: 9887100

注意事項

For research use only.

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参考文献

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