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Anti-Human HIF1A Recombinant Antibody (SAA0814) (HS858013)

Anti-Human HIF1A Recombinant Antibody (SAA0814)
Anti-Human HIF1A Recombinant Antibody (SAA0814)
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Overview
Catalog No.HS858013
Species reactivityHuman
ApplicationsELISA, FCM, IP, WB
Host speciesMouse
IsotypeVHH-mFc
Clone IDSAA0814
Clonality Monoclonal
Target HIF1A, MOP1, bHLHe78, Basic-helix-loop-helix-PAS protein MOP1, HIF-1-alpha, BHLHE78, Member of PAS protein 1, Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, PAS domain-containing protein 8, HIF1-alpha, PASD8, Class E basic helix-loop-helix protein 78
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q16665
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-Human HIF1A Recombinant Antibody (SAA0814)

    Flow Cytometry

    Flow-cytometry using anti-human HIF1A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human HIF1A antibody monoclonal antibody (Catalog # HS858013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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