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| カタログ番号 | HM814107 |
|---|---|
| 種反応性 | Human |
| アプリケーション | FCM |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | H4H2 |
| ターゲット | HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, HLAB, HLA-B*27 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P01889 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | HLA class I histocompatibility antigen, B alpha chain (HLA-B*27) is a ~40 kDa protein. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-B-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells. May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity. Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells. Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via constitutive proteasome and IFNG-induced immunoproteasome. 1. Schmidt, J. et al. (2012) PLoS pathogens 8, e1003042. PMID: 23209413 2. Kløverpris, HN. et al. (2015) Retrovirology 12, 20. PMID: 25808313 3. Chan, KF. et al. (2018) Nature communications 9, 1026. PMID: 29531227 4. Peh, CA. et al. (1998) Immunity 8, 531-42. PMID: 9620674 5. Hillen, N. et al. (2008) European journal of immunology 38, 2993-3003. PMID: 18991276 6. Barber, LD. et al. (1995) Current biology : CB 5, 179-90. PMID: 7743181 7. Motozono, C. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 3428-34. PMID: 24600035 |
| 注意事項 | For research use only. |
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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