Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

Name: Anti-Human HRAS Recombinant Antibody (Y13-259)
Brand: abinScience
SKU: HF778023
Price: 110 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HF778023

Reactividad de especies

Human

Aplicaciones

IF, IP, WB

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

Y13-259

Objetivo

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P01112

RRID

Anti-Human HRAS Antibody (Y13-259) (abinScience Cat# HF778023, RRID:AB_3728964)
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Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

GTPase HRas is a ~21 kDa protein. Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

1. Gripp, KW. et al. (2012) American journal of medical genetics. Part A 158A, 2106-18. PMID: 22821884

2. Williams, JG. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6376-81. PMID: 12740440

3. Guil, S. et al. (2003) Cancer research 63, 5178-87. PMID: 14500341

4. Lander, HM. et al. (1997) The Journal of biological chemistry 272, 4323-6. PMID: 9020151

Nota

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).
Flow CytoMetry

Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng
Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng