Anti-HRAS Polyclonal Antibody (HF778014)

Name: Anti-HRAS Polyclonal Antibody
Brand: abinScience
SKU: HF778014
Price: 168 USD
Availability: InStock

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Datasheet
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Overview

Catalog No.

HF778014

Description

Anti-HRAS Polyclonal Antibody (HF778014) is a rabbit polyclonal antibody detecting HRAS in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Cyprinus carpio, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HRAS (Phe82-Glu176).

Target

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Purification

Purified by antigen affinity column.

Accession

P01112

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

GTPase HRas is a ~21 kDa protein. Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

1. Gripp, KW. et al. (2012) American journal of medical genetics. Part A 158A, 2106-18. PMID: 22821884

2. Williams, JG. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6376-81. PMID: 12740440

3. Guil, S. et al. (2003) Cancer research 63, 5178-87. PMID: 14500341

4. Lander, HM. et al. (1997) The Journal of biological chemistry 272, 4323-6. PMID: 9020151

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HRAS polyclonal antibody at 1ug/mL on recombinant HRAS (Catalog No. HF778012).

References

Datasheet

Anti-HRAS Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HRAS Polyclonal Antibody (HF778014)

Overview

Images

References

Datasheet

SDS

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