Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

Name: Anti-Human HRAS Recombinant Antibody (Y13-259)
Brand: abinScience
SKU: HF778023
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF778023

Species reactivity

Human

Applications

IF, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

Y13-259

Clonality

Monoclonal

Target

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01112

RRID

Anti-Human HRAS Antibody (Y13-259) (abinScience Cat# HF778023, RRID:AB_3728964)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

GTPase HRas is a ~21 kDa protein. Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

1. Gripp, KW. et al. (2012) American journal of medical genetics. Part A 158A, 2106-18. PMID: 22821884

2. Williams, JG. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6376-81. PMID: 12740440

3. Guil, S. et al. (2003) Cancer research 63, 5178-87. PMID: 14500341

4. Lander, HM. et al. (1997) The Journal of biological chemistry 272, 4323-6. PMID: 9020151

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).
Flow CytoMetry

Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng
Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng