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Anti-Human HTR4 Polyclonal Antibody (HC684014)

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Vista general
Número de catálogoHC684014
Descripción
Anti-Human HTR4 Polyclonal Antibody (HC684014) is a rabbit polyclonal antibody detecting HTR4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HTR4 (Arg214-Thr260).
Objetivo HTR4, Serotonin receptor 4, 5-HT-4, 5-HT4, 5-hydroxytryptamine receptor 4
Purificación Purified by antigen affinity column.
Número de acceso Q13639
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

5-hydroxytryptamine receptor 4 (HTR4) is a ~43 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.

1. Mialet, J. et al. (2000) British journal of pharmacology 130, 527-38. PMID: 10821780
2. Kamel, R. et al. (2005) Biochemical pharmacology 70, 1009-18. PMID: 16102731
3. Huang, S. et al. (2022) Molecular cell 82, 2681-2695.e6. PMID: 35714614
4. Blondel, O. et al. (1998) Journal of neurochemistry 70, 2252-61. PMID: 9603189
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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