Anti-Human Jo-1/HARS1 Polyclonal Antibody (HY584024)

Name: Anti-Human Jo-1/HARS1 Polyclonal Antibody
Brand: abinScience
SKU: HY584024
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY584024

説明

Anti-Human jo-1 Polyclonal Antibody (HY584024) is a rabbit polyclonal antibody detecting jo-1 in ELISA, IHC, WB. Suitable for Mus musculus, Human, Pongo abelii, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human jo-1 (Met1-Cys509).

ターゲット

jo-1

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Purified by antigen affinity column.

アクセッション番号

P12081

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

別名

Histidine--tRNA ligase, cytoplasmic, 6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS

背景

Histidine--tRNA ligase, cytoplasmic (jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198

2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-HARS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT116 cell lysate
Lane 6: Jurkat cell lysate

参考文献

データシート

Anti-Human Jo-1/HARS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human Jo-1/HARS1 Polyclonal Antibody (HY584024)

概要

イメージ

参考文献

データシート

SDS