Anti-Human KPNB1 Polyclonal Antibody (HD617014)

Name: Anti-Human KPNB1 Polyclonal Antibody
Brand: abinScience
SKU: HD617014
Price: 168 USD
Availability: InStock

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Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HD617014

Description

Anti-Human KPNB1 Polyclonal Antibody (HD617014) is a rabbit polyclonal antibody detecting KPNB1. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human KPNB1 (Met1-Ala442).

Cible

Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q14974

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

Importin subunit beta-1 (KPNB1) is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 10228156

2. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 11682607

3. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 11891849

4. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 19386897

5. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 20818336

6. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 24699649

7. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 7615630

8. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-KPNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A2780s cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Hela cell lysate
Lane 4: K562 cell lysate
Lane 5: A549 cell lysate
Lane 6: Caco2 cell lysate

Reférences

Fiche technique

Anti-Human KPNB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human KPNB1 Polyclonal Antibody (HD617014)

Aperçu

Images

Reférences

Fiche technique

SDS