Human KPNB1 Recombinant Protein (N-His) (HD617012)

Name: Human KPNB1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD617012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HD617012
Description	Recombinant Human KPNB1 Protein, N-His (HD617012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q14974
Protein length	Met1-Ala442
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit
Background	Importin subunit beta-1 is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 10228156 2. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 11682607 3. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 11891849 4. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 19386897 5. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 20818336 6. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 24699649 7. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 7615630 8. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515
Note	For research use only

Images

References

Datasheet

Human KPNB1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human KPNB1 Recombinant Protein (N-His) (HD617012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us