Anti-Human KPNB1 Polyclonal Antibody (HD617014)

Name: Anti-Human KPNB1 Polyclonal Antibody
Brand: abinScience
SKU: HD617014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD617014

Description

Anti-Human KPNB1 Polyclonal Antibody (HD617014) is a rabbit polyclonal antibody detecting KPNB1. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KPNB1 (Met1-Ala442).

Target

Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14974

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Importin subunit beta-1 (KPNB1) is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 10228156

2. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 11682607

3. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 11891849

4. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 19386897

5. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 20818336

6. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 24699649

7. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 7615630

8. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-KPNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A2780s cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Hela cell lysate
Lane 4: K562 cell lysate
Lane 5: A549 cell lysate
Lane 6: Caco2 cell lysate

References

Datasheet

Anti-Human KPNB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human KPNB1 Polyclonal Antibody (HD617014)

Overview

Images

References

Datasheet

SDS

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