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| カタログ番号 | HD617014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human KPNB1 Polyclonal Antibody (HD617014) is a rabbit polyclonal antibody detecting KPNB1. Suitable for Mouse, Rat, and Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human KPNB1 (Met1-Ala442). | ||||||||
| ターゲット | Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q14974 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 背景 | Importin subunit beta-1 (KPNB1) is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 10228156 2. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 11682607 3. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 11891849 4. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 19386897 5. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 20818336 6. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 24699649 7. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 7615630 8. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-KPNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A2780s cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Hela cell lysate
Lane 4: K562 cell lysate
Lane 5: A549 cell lysate
Lane 6: Caco2 cell lysate
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