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Anti-Human LPL/LIPD Recombinant Antibody (5D2), FITC (HY363117)

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Обзор
Каталожный номерHY363117
БрендabinScience
Реактивность видовHuman, Mouse, Rat, Bovine
ПримененияELISA, FCM, IF(P), WB
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG2a
ID клона5D2
КонъюгацияFITC
Цель Phospholipase A1, LIPD, Lipoprotein lipase, LPL
Уровень эндотоксинов Please contact with the lab for this information.
Связанные продукты Mouse IgG2a Recombinant Isotype Control Antibody (C1.18.4), FITC
Номер доступа P06858
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

Lipoprotein lipase (LPL/LIPD) is a ~53 kDa protein. Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.

1. Lutz, EP. et al. (2001) The Journal of clinical investigation 107, 1183-92. PMID: 11342582
2. Pingitore, P. et al. (2016) Journal of clinical lipidology 10, 816-823. PMID: 27578112
3. Weinstock, PH. et al. (1995) The Journal of clinical investigation 96, 2555-68. PMID: 8675619
4. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167
5. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706
6. Goulbourne, CN. et al. (2014) Cell metabolism 19, 849-60. PMID: 24726386
7. Allan, CM. et al. (2017) Journal of lipid research 58, 216-225. PMID: 27811232
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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