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Anti-Human MAGEA6 Polyclonal Antibody (HW297014)

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Anti-Human MAGEA6 Polyclonal Antibody
Anti-Human MAGEA6 Polyclonal Antibody
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Aperçu
Numéro de catalogueHW297014
Description
Anti-Human MAGEA6 Polyclonal Antibody (HW297014) is a rabbit polyclonal antibody detecting MAGEA6 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human MAGEA6 (Ser96-Glu314).
Cible MAGEA6, Cancer/testis antigen 1.6, MAGE6, MAGE3B antigen, MAGE-6 antigen, CT1.6, Melanoma-associated antigen 6
Purification Purified by antigen affinity column.
Numéro d'accès P43360
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Melanoma-associated antigen 6 (MAGEA6) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928
2. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041
3. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705
Note For research use only.
Images
  • Anti-Human MAGEA6 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MAGEA6 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse testis lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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