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Anti-Human MC3R Polyclonal Antibody (HC053014)

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Aperçu
Numéro de catalogueHC053014
Description
Anti-Human MC3R Polyclonal Antibody (HC053014) is a rabbit polyclonal antibody detecting Melanocortin receptor 3; MC3-R; MC3R in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human MC3R (Asn2-Gln37&Val101-Asn118&Arg302-Gly323).
Cible Melanocortin receptor 3; MC3-R; MC3R
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P41968
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Melanocortin receptor 3 (MC3R) is a ~36 kDa protein. G protein-coupled receptor for melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor. Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which contributes to the regulation of energy homeostasis. Required for expression of anticipatory patterns of activity and wakefulness during periods of limited nutrient availability and for the normal regulation of circadian clock activity in the brain. Binding of the Agouti-related protein/AGPR antagonist precludes alpha-MSH-induced signaling, blocking cAMP production.

1. Feng, W. et al. (2023) Cell discovery 9, 81. PMID: 37524700
2. Gantz, I. et al. (1993) The Journal of biological chemistry 268, 8246-50. PMID: 8463333
3. Mencarelli, M. et al. (2008) European journal of human genetics : EJHG 16, 581-6. PMID: 18231126
4. Ollmann, MM. et al. (1997) Science (New York, N.Y.) 278, 135-8. PMID: 9311920
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human MC3R Polyclonal Antibody

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