Human MC3R Recombinant Protein (N-His-KSI) (HC053012)

Name: Human MC3R Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HC053012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC053012
Description	Recombinant Human MC3R Protein, N-His-KSI (HC053012) expressed in E. coli. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P41968
Protein length	Asn2-Gln37&Val101-Asn118&Arg302-Gly323
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Melanocortin receptor 3; MC3-R; MC3R
Background	Melanocortin receptor 3 is a ~36 kDa protein. G protein-coupled receptor for melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor. Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which contributes to the regulation of energy homeostasis. Required for expression of anticipatory patterns of activity and wakefulness during periods of limited nutrient availability and for the normal regulation of circadian clock activity in the brain. Binding of the Agouti-related protein/AGPR antagonist precludes alpha-MSH-induced signaling, blocking cAMP production. 1. Feng, W. et al. (2023) Cell discovery 9, 81. PMID: 37524700 2. Gantz, I. et al. (1993) The Journal of biological chemistry 268, 8246-50. PMID: 8463333 3. Mencarelli, M. et al. (2008) European journal of human genetics : EJHG 16, 581-6. PMID: 18231126 4. Ollmann, MM. et al. (1997) Science (New York, N.Y.) 278, 135-8. PMID: 9311920
Note	For research use only

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SDS-PAGE for Recombinant Human MC3R Protein, N-His-KSI

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Datasheet

Human MC3R Recombinant Protein (N-His-KSI).pdf

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Human MC3R Recombinant Protein (N-His-KSI) (HC053012)

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