Anti-Human MDA5 Recombinant Antibody (SAA2092) (HV365023)

Name: Anti-Human MDA5 Recombinant Antibody (SAA2092)
Brand: abinScience
SKU: HV365023
Price: 196 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV365023

Reactivité des espèces

Human

Applications

ELISA, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

SAA2092

Cible

MDA-5, IFIH1, Helicard, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Interferon-induced with helicase C domain protein 1, Helicase with 2 CARD domains, RNA helicase-DEAD box protein 116, RIG-I-like receptor 2, Interferon-induced helicase C domain-containing protein 1, MDA5, Murabutide down-regulated protein, Melanoma differentiation-associated protein 5, CADM-140 autoantigen, RH116, RLR-2

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q9BYX4

RRID

Anti-Human MDA5/IFIH1 Antibody (SAA2092) (abinScience Cat# HV365023, RRID:AB_3729352)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Interferon-induced helicase C domain-containing protein 1 (MDA5/IFIH1) is a ~116 kDa protein. Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2.

1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402

2. Wu, XM. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 204, 2216-2231. PMID: 32169843

3. Liu, G. et al. (2021) Nature microbiology 6, 467-478. PMID: 33727702

4. Peisley, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 21010-5. PMID: 22160685

5. Lamborn, IT. et al. (2017) The Journal of experimental medicine 214, 1949-1972. PMID: 28606988

6. Yin, X. et al. (2021) Cell reports 34, 108628. PMID: 33440148

7. Rebendenne, A. et al. (2021) Journal of virology 95. PMID: 33514628

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MDA5/IFIH1 Antibody (SAA2092).

Reférences

Fiche technique

Anti-Human MDA5 Recombinant Antibody (SAA2092).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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