Anti-Human MDA5 Polyclonal Antibody (HV365024)

Name: Anti-Human MDA5 Polyclonal Antibody
Brand: abinScience
SKU: HV365024
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV365024

Description

Anti-Human MDA5/IFIH1 Polyclonal Antibody (HV365024) is a rabbit polyclonal antibody detecting IFIH1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MDA5/IFIH1 (Arg309-Asp1025).

Target

MDA-5, IFIH1, Helicard, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Interferon-induced with helicase C domain protein 1, Helicase with 2 CARD domains, RNA helicase-DEAD box protein 116, RIG-I-like receptor 2, Interferon-induced helicase C domain-containing protein 1, MDA5, Murabutide down-regulated protein, Melanoma differentiation-associated protein 5, CADM-140 autoantigen, RH116, RLR-2

Purification

Purified by antigen affinity column.

Accession

Q9BYX4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon-induced helicase C domain-containing protein 1 (MDA5/IFIH1) is a ~116 kDa protein. Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2.

1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402

2. Wu, XM. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 204, 2216-2231. PMID: 32169843

3. Liu, G. et al. (2021) Nature microbiology 6, 467-478. PMID: 33727702

4. Peisley, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 21010-5. PMID: 22160685

5. Lamborn, IT. et al. (2017) The Journal of experimental medicine 214, 1949-1972. PMID: 28606988

6. Yin, X. et al. (2021) Cell reports 34, 108628. PMID: 33440148

7. Rebendenne, A. et al. (2021) Journal of virology 95. PMID: 33514628

Note

For research use only.

Images

References

Datasheet

Anti-Human MDA5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human MDA5 Polyclonal Antibody (HV365024)

Overview

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References

Datasheet

SDS

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