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| カタログ番号 | HV365033 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, WB | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1, kappa | ||||||
| クローンID | SAA2313 | ||||||
| テスト済みアプリケーション | WB | ||||||
| ターゲット | Interferon-induced helicase C domain-containing protein 1, EC:3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116, IFIH1, MDA5, RH116 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | Q9BYX4 | ||||||
| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 背景 | Interferon-induced helicase C domain-containing protein 1 (MDA5/IFIH1) is a ~116 kDa protein. Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2. 1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402 2. Wu, XM. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 204, 2216-2231. PMID: 32169843 3. Liu, G. et al. (2021) Nature microbiology 6, 467-478. PMID: 33727702 4. Peisley, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 21010-5. PMID: 22160685 5. Lamborn, IT. et al. (2017) The Journal of experimental medicine 214, 1949-1972. PMID: 28606988 6. Yin, X. et al. (2021) Cell reports 34, 108628. PMID: 33440148 7. Rebendenne, A. et al. (2021) Journal of virology 95. PMID: 33514628 | ||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-MDA5 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MDA5 (Catalog No: HV365021)
Lane 2: negative control
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