Anti-Human MDA5 Recombinant Antibody (SAA2314) (HV365043)

Name: Anti-Human MDA5 Recombinant Antibody (SAA2314)
Brand: abinScience
SKU: HV365043
Price: 126 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV365043

Reactividad de especies

Human

Aplicaciones

ELISA, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

SAA2314

Aplicaciones probadas

Objetivo

Interferon-induced helicase C domain-containing protein 1, EC:3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116, IFIH1, MDA5, RH116

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q9BYX4

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Interferon-induced helicase C domain-containing protein 1 (MDA5/IFIH1) is a ~116 kDa protein. Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2.

1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402

2. Wu, XM. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 204, 2216-2231. PMID: 32169843

3. Liu, G. et al. (2021) Nature microbiology 6, 467-478. PMID: 33727702

4. Peisley, A. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 21010-5. PMID: 22160685

5. Lamborn, IT. et al. (2017) The Journal of experimental medicine 214, 1949-1972. PMID: 28606988

6. Yin, X. et al. (2021) Cell reports 34, 108628. PMID: 33440148

7. Rebendenne, A. et al. (2021) Journal of virology 95. PMID: 33514628

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-MDA5 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MDA5 (Catalog No: HV365021)
Lane 2: negative control

Referencias

Datasheet

Anti-Human MDA5 Recombinant Antibody (SAA2314).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo