Anti-Human MICA/B Recombinant Antibody (20C6) (HS894013)

Name: Anti-Human MICA/B Recombinant Antibody (20C6)
Brand: abinScience
SKU: HS894013
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HS894013

Reactividad de especies

Human

Aplicaciones

ELISA

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

20C6

Aplicaciones probadas

FCM

Objetivo

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MIC-B, MHC class I polypeptide-related sequence B, MICB, PERB11.2

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q29983, Q29980

RRID

Anti-Human MICA/B Antibody (20C6) (abinScience Cat# HS894013, RRID:AB_3726911)
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Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

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1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531

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2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960

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3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human MICA/B Antibody (20C6).
Flow CytoMetry

Flow-cytometry using anti-human MICA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MICA monoclonal antibody (Catalog: HS894013) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.