Anti-Human MICA/B Recombinant Antibody (20C6) (HS894013)

Name: Anti-Human MICA/B Recombinant Antibody (20C6)
Brand: abinScience
SKU: HS894013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HS894013

Reactivité des espèces

Human

Applications

ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

20C6

Applications testées

FCM

Cible

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MIC-B, MHC class I polypeptide-related sequence B, MICB, PERB11.2

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q29983, Q29980

RRID

Anti-Human MICA/B Antibody (20C6) (abinScience Cat# HS894013, RRID:AB_3726911)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

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1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531

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2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960

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3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MICA/B Antibody (20C6).
Flow CytoMetry

Flow-cytometry using anti-human MICA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MICA monoclonal antibody (Catalog: HS894013) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.