Anti-Human MICA/B Recombinant Antibody (20C6) (HS894013)

Name: Anti-Human MICA/B Recombinant Antibody (20C6)
Brand: abinScience
SKU: HS894013
Price: 191 USD
Availability: InStock

データシート製品共有情報コピー

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50ug
100ug
1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HS894013

種反応性

Human

アプリケーション

ELISA

宿主種

Mouse

クローン性

Monoclonal

アイソタイプ

IgG2a, kappa

クローンID

20C6

テスト済みアプリケーション

FCM

ターゲット

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MIC-B, MHC class I polypeptide-related sequence B, MICB, PERB11.2

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

Q29983, Q29980

RRID

Anti-Human MICA/B Antibody (20C6) (abinScience Cat# HS894013, RRID:AB_3726911)
_x005F_x005F_x000D_

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

背景

MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

_x005F_x005F_x000D_ _x005F_x005F_x000D_

1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531

_x005F_x005F_x000D_ _x005F_x005F_x000D_

2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960

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3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

注意事項

For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Anti-Human MICA/B Antibody (20C6).
Flow CytoMetry

Flow-cytometry using anti-human MICA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MICA monoclonal antibody (Catalog: HS894013) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.