Anti-Human MMP-14/MT1-MMP Recombinant Antibody (LEM-2/15) (HW299013)

Name: Anti-Human MMP-14/MT1-MMP Recombinant Antibody (LEM-2/15)
Brand: abinScience
SKU: HW299013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW299013

Reactividad de especies

Human

Aplicaciones

Blocking, ELISA, ICC, IF, FCM, WB

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

LEM-2/15

Objetivo

MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P50281

RRID

Anti-Human MMP14 Antibody (LEM-2/15) (abinScience Cat# HW299013, RRID:AB_3725683)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Matrix metalloproteinase-14 (MMP14) is a ~65 kDa protein. Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7.

1. Sato, H. et al. (1994) Nature 370, 61-5. PMID: 8015608

2. Gu, G. et al. (2012) Journal of cellular biochemistry 113, 1013-21. PMID: 22065321

3. Noda, K. et al. (2003) Investigative ophthalmology & visual science 44, 2163-70. PMID: 12714657

4. Golubkov, VS. et al. (2010) The Journal of biological chemistry 285, 35740-9. PMID: 20837484

5. Jin, G. et al. (2011) The EMBO journal 30, 2281-93. PMID: 21572390

6. Cork, SM. et al. (2012) Oncogene 31, 5144-52. PMID: 22330140

7. Chow, CFW. et al. (2022) Nature metabolism 4, 203-212. PMID: 35177851

Nota

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Imágenes

Bioactivity

Detects MMP14 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15).
Flow Cytometry

Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MMP14 (Catalog No: HW299022)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 monoclonal antibody (Catalog HW299013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.