Anti-Human MMP-14/MT1-MMP Recombinant Antibody (LEM-2/15) (HW299013)

Name: Anti-Human MMP-14/MT1-MMP Recombinant Antibody (LEM-2/15)
Brand: abinScience
SKU: HW299013
Price: 126 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HW299013

Реактивность видов

Human

Применения

Blocking, ELISA, ICC, IF, FCM, WB

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

LEM-2/15

Цель

MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P50281

RRID

Anti-Human MMP14 Antibody (LEM-2/15) (abinScience Cat# HW299013, RRID:AB_3725683)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Matrix metalloproteinase-14 (MMP14) is a ~65 kDa protein. Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7.

1. Sato, H. et al. (1994) Nature 370, 61-5. PMID: 8015608

2. Gu, G. et al. (2012) Journal of cellular biochemistry 113, 1013-21. PMID: 22065321

3. Noda, K. et al. (2003) Investigative ophthalmology & visual science 44, 2163-70. PMID: 12714657

4. Golubkov, VS. et al. (2010) The Journal of biological chemistry 285, 35740-9. PMID: 20837484

5. Jin, G. et al. (2011) The EMBO journal 30, 2281-93. PMID: 21572390

6. Cork, SM. et al. (2012) Oncogene 31, 5144-52. PMID: 22330140

7. Chow, CFW. et al. (2022) Nature metabolism 4, 203-212. PMID: 35177851

Примечание

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Изображения

Bioactivity

Detects MMP14 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15).
Flow Cytometry

Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MMP14 (Catalog No: HW299022)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 monoclonal antibody (Catalog HW299013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.