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Anti-Human MMP-9 Recombinant Antibody (SAA0116) (HB945107)

Anti-Human MMP-9 Recombinant Antibody (SAA0116)
Anti-Human MMP-9 Recombinant Antibody (SAA0116)
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Aperçu
Numéro de catalogueHB945107
Reactivité des espècesHuman, Rat
ApplicationsELISA, FCM, IHC
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG4, kappa
ID de cloneSAA0116
Applications testées FCM
Cible Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P14780
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Matrix metalloproteinase-9 (MMP9) is a ~78 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.

1. Takino, T. et al. (2003) Oncogene 22, 4617-26. PMID: 12879005
2. Tschesche, H. et al. (1992) Matrix (Stuttgart, Germany). Supplement 1, 245-55. PMID: 1480034
3. Wilhelm, SM. et al. (1989) The Journal of biological chemistry 264, 17213-21. PMID: 2551898
4. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094
Note For research use only.
Images
  • Anti-Human MMP-9 Recombinant Antibody (SAA0116)

    Flow Cytometry

    Flow-cytometry using anti-human MMP9 antibody.MMP9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP9 antibody monoclonal antibody (Catalog # HB945107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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