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Обзор
Каталожный номерHD517014
Описание
Anti-Human NAPEPLD Polyclonal Antibody (HD517014) is a rabbit polyclonal antibody detecting NAPEPLD. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human NAPEPLD (Ser76-Phe393).
Цель C7orf18, EC:3.1.4.54, N-acyl phosphatidylethanolamine phospholipase D, N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, NAPE-PLD, NAPE-hydrolyzing phospholipase D, NAPEPLD
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q6IQ20
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) is a ~45 kDa protein. D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose.

1. Okamoto, Y. et al. (2004) The Journal of biological chemistry 279, 5298-305. PMID: 14634025
2. Wang, J. et al. (2006) The Journal of biological chemistry 281, 12325-35. PMID: 16527816
3. Magotti, P. et al. (2015) Structure (London, England : 1993) 23, 598-604. PMID: 25684574
4. Margheritis, E. et al. (2016) ACS chemical biology 11, 2908-2914. PMID: 27571266
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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