Human NAPEPLD Recombinant Protein (N-His) (HD517012)

Name: Human NAPEPLD Recombinant Protein (N-His)
Brand: abinScience
SKU: HD517012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD517012
Description	Recombinant Human NAPEPLD Protein, N-His (HD517012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q6IQ20
Protein length	Ser76-Phe393
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	C7orf18, EC:3.1.4.54, N-acyl phosphatidylethanolamine phospholipase D, N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, NAPE-PLD, NAPE-hydrolyzing phospholipase D, NAPEPLD
Background	N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D is a ~45 kDa protein. D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. 1. Okamoto, Y. et al. (2004) The Journal of biological chemistry 279, 5298-305. PMID: 14634025 2. Wang, J. et al. (2006) The Journal of biological chemistry 281, 12325-35. PMID: 16527816 3. Magotti, P. et al. (2015) Structure (London, England : 1993) 23, 598-604. PMID: 25684574 4. Margheritis, E. et al. (2016) ACS chemical biology 11, 2908-2914. PMID: 27571266
Note	For research use only

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Datasheet

Human NAPEPLD Recombinant Protein (N-His).pdf

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Human NAPEPLD Recombinant Protein (N-His) (HD517012)

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