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Anti-Human NAPEPLD Polyclonal Antibody (HD517014)

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Overview
Catalog No.HD517014
Description
Anti-Human NAPEPLD Polyclonal Antibody (HD517014) is a rabbit polyclonal antibody detecting NAPEPLD. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NAPEPLD (Ser76-Phe393).
Target C7orf18, EC:3.1.4.54, N-acyl phosphatidylethanolamine phospholipase D, N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, NAPE-PLD, NAPE-hydrolyzing phospholipase D, NAPEPLD
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6IQ20
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) is a ~45 kDa protein. D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose.

1. Okamoto, Y. et al. (2004) The Journal of biological chemistry 279, 5298-305. PMID: 14634025
2. Wang, J. et al. (2006) The Journal of biological chemistry 281, 12325-35. PMID: 16527816
3. Magotti, P. et al. (2015) Structure (London, England : 1993) 23, 598-604. PMID: 25684574
4. Margheritis, E. et al. (2016) ACS chemical biology 11, 2908-2914. PMID: 27571266
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human NAPEPLD Polyclonal Antibody

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