Anti-Human NELFE Polyclonal Antibody (HB121014)

Name: Anti-Human NELFE Polyclonal Antibody
Brand: abinScience
SKU: HB121014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HB121014

Description

Anti-Human NELFE Polyclonal Antibody (HB121014) is a rabbit polyclonal antibody detecting NELFE in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NELFE (Gly261-Arg331).

Cible

RDBP, RD, NELF-E, RNA-binding protein RD, NELFE, Negative elongation factor E

Purification

Purified by antigen affinity column.

Numéro d'accès

P18615

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Negative elongation factor E (NELFE) is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.

1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401

2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882

3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650

4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062

5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858

6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human NELFE Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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