Human NELFE Recombinant Protein (N-His) (HB121022)

Name: Human NELFE Recombinant Protein (N-His)
Brand: abinScience
SKU: HB121022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB121022
Description	Recombinant Human NELFE Protein, N-His (HB121022) expressed in E. coli, spanning Ser249-Phe380. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P18615
Protein length	Ser249-Phe380
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.96 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NELF-E, NELFE, Negative elongation factor E, RD, RDBP, RNA-binding protein RD
Background	Negative elongation factor E is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. 1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882 3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650 4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062 5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858 6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391
Note	For research use only

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Datasheet

Human NELFE Recombinant Protein (N-His).pdf

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Human NELFE Recombinant Protein (N-His) (HB121022)

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