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Anti-Human NELFE Polyclonal Antibody (HB121014)

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Overview
Catalog No.HB121014
Description
Anti-Human NELFE Polyclonal Antibody (HB121014) is a rabbit polyclonal antibody detecting NELFE in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NELFE (Gly261-Arg331).
Target RDBP, RD, NELF-E, RNA-binding protein RD, NELFE, Negative elongation factor E
Purification Purified by antigen affinity column.
Accession P18615
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Negative elongation factor E (NELFE) is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.

1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401
2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882
3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650
4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062
5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858
6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human NELFE Polyclonal Antibody

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